Development of a Functional Marker for Rice Blast Resistance Gene Pikh and Natural Variation at Pikh Locus in japonica Rice in Jiangsu Province， China

doi:10.3969/j.issn.1001-7216.2014.02.005

Abstract

Abstract: Pikhis a vital non racespecific resistance gene for rice blast. In this study, one InDel marker, FM143 was developed based on the 143 bp insertion between the susceptible and resistant Pikh alleles. Previous studies showed that Tetep carried resistant Pikh allele and had the noneinsertion genotype for PCR by FM143. F2 population, derived from Tetep and Ning 9108, were detected by marker FM143 and the result indicated that FM143 could effectively identify Pikh/Pikh, Pikh/pikh and pikh/pikh genotypes with the expected segregation ratio 1∶2∶1. Sixtyfive japonica varieties widely grown in Jiangsu Province over years and sixtyfour japonica lines were genotyped by FM143. Among them, 19 japonica varieties and 22 japonica lines showed noneinsertion genotype. Further sequencing at the target Pikh locus for these noneinsertion genotype showed that there was one highly conserved singlebase mutation (G→C) at 102 bp location compared with the whole target gene sequence in Tetep and public Pikh GeneBank leading to the changes from glutamic acid to asparaginic acid. This new allele in Jiangsu japonica rice was termed as PikhJSJ.

Key words: rice, rice blast, Pikh, functional marker, PikhJSJ

摘要： 稻瘟病抗病基因Pikh是对稻瘟病菌具有广谱抗性的一个重要基因。根据Pikh感病基因和抗病基因序列存在143bp 插入的特征，设计了InDel 标记FM143。Tetep具有抗病基因Pikh，分子标记FM143 PCR 扩增带型为非插入带型，而不携带Pikh的材料的带型为插入带型。利用该标记对Tetep与宁9108的F2 群体进行分析，结果表明该标记能准确区分Pikh、Pikh纯合及杂合基因型，标记基因型符合1∶2∶1比例，没有发生偏分离现象。利用该标记对65份江苏省历年来大面积推广的粳稻品种和64 份粳稻品系进行检测，筛选出具有非插入带型的粳稻品种19份、粳稻品系22份。进一步对江苏省粳稻品种中Pikh位点进行测序，发现江苏粳稻品种（系）Pikh基因座位与Tetep和已经登录的Pikh序列相比在102 bp处存在一个单碱基的变异（由G突变为C），使得谷氨酸变异为天门冬氨酸，且该变异高度保守。将江苏省粳稻品种中发现的Pikh位点的等位基因暂定名为PikhJSJ。

关键词: 水稻, 稻瘟病, Pikh, 功能标记, PikhJSJ

CLC Number:

WANG Jun, YANG Jie, ZHU Jinyan, FAN Fangjun, LI Wenqi, WANG Fangquan, HUANG Zhuanyun, . Development of a Functional Marker for Rice Blast Resistance Gene Pikh and Natural Variation at Pikh Locus in japonica Rice in Jiangsu Province， China[J]. Chinese Journal of Rice Science, 2014, 28(2): 141-147.

王军,杨杰,朱金燕,范方军,李文奇,王芳权,黄转运,仲维功*. 稻瘟病抗病基因Pi-k^h功能标记的开发及江苏粳稻品种中Pi-k^h的变异[J]. 中国水稻科学, 2014, 28(2): 141-147.

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